10 mM Buffer DNA 10 uM ascorbic inhibitor of stock 2 Tube or

10 mM Buffer DNA 10 uM ascorbic inhibitor of stock 2 Tube (or (100 mM Na Contents Label pH 7.4) choice) C Control (plasmid only) L5 HL 15 HL 5 HL 3 ascorbic acid 12 HL AA CuCl2 Cu 14.5 HL EDTA/ EDTA (or other inhibitor) 5 uL 2 uL 13 uL inhibitor 11.5 uL 1 ascorbic acid/ CuCl2 (0.25 HM) 5 uL 0.5 uL 3 uL ascorbic acid/ CuCl2 (1 HM) 5 uL 2 uL 3 uL 10 uL ascorbic acid/ Cucl2 (5 HM) L5 uL 8 HL 3 HL ascorbic acid/ CuCl2 (1 HM) 2 uL 3 uL 8 uL /EDTA

Solution

To some extent the concentration of EDTA, Ascorbic acid, and CuCl₂ protects the damage to both single as well as the double stranded breaks.