Compared to the proteins in the trans Golgi compartment thos
Solution
contain fewer modifications
Proteins destined to be secreted from the eukaryotic cell are synthesized by ribosomes bound to the rough endoplasmic reticulum (RER). As the protein is synthesized, it is translocated across the RER membrane into the lumen of the RER where it folds into its final conformation. The ER then buds off vesicles that carry the protein to the golgi apparatus also called the golgi complex.
Glycosylation: First, N-acetylgalactosamine (GalNAc) is transferred to the relevant Ser or Thr residue of the protein by GalNAc transferase, an enzyme that uses UDP-GalNAc as the precursor. Other monosaccharides (galactose, N-acetylglucosamine (GlcNAc), sialic acid, fucose) are then added using the corresponding sugar nucleotides as precursors. The exact type and number (up to about 10) of monosaccharides added depends on the protein substrate.
