You are about to mix DN A to run on an agarose gel You need
You are about to mix DN A to run on an agarose gel. You need a told) of 5 mu to load into each well in the agarose gel. You need to mix DNA with loading dye so that it can be visualized while the gel is running. The loading dye comes as n 5X concentrate. The concentration of DNA you have how much of DNA. loading dye and sterile Dl water you will need to prepare a mixture that with contain -1.5 5 mu g of DNA
Solution
Mix 1.5µl of DNA, 2.5 µl of loading buffer and 1µl of sterile distilled water in a 1ml eppendorf tubes. From this, take 5µl of sample and load into the agarose gel.
