Please explain clearly You also perform PCR on the the betag

Please explain clearly.

You also perform PCR on the the beta-globin gene using genomic DNA as the template. Below is shown a 1, 600 base pair (bp) genomic Beta-globin PCR product and a 2,000 bp plasmid you want to insert it into. Positions of important restriction sites are shown. You cut both of he PCR product and the plasmid with BamHI and EcoRI and ligate them together. After transforming the ligated plasmid into E. coli, you isolate the plasmid and cut it with Xbal. List the sizes of the DNA fragments you expect? (3) The schematic of the plasmid shows three important regions: \'bla\' is a gene encoding beta-lactamase which inactivates Ampicilin, \'poly\' is the polylinker, and \'ori\' is an origin of replication.

Solution

When inserted in the plasmid by using BamH1 and EcoR1 digestion the fragment of 1-10 bp is released and 1600bp is added which comes from the gene. And as gene itself has Xba1 site in itself the expected size of fragments when cut with Xba1 are

1. 1000bp +1980 bp will come from the gene and vector respectively.

2. 600+10 bp from gene and vector.