Please answer all of 4 The pRb protein is regulated by coval

The pRb protein is regulated by covalent modification. Phosphorylation of the pRb prevents its association with E2F. During which phase of the cell cycle is pRb first phosphorylated and by which cydin-CDK complex? What consequence does phosphorylation of pRB have on cell cycle progression? After the completion of S phase, E2F function is no longer needed. pRb must be dephosphorylated so that it can once again bind to E2F. Name the type of enzyme responsible for removing phosphate groups from proteins. Knowing what you know about the cell cycle, explain why the RB gene would be considered a tumor suppressor gene.

Solution

A) 1. Cyclin-dependent kinase complex(CDKC) or (cyclin-CDK) is a protein compound organized by the confederation of an unenergetic catalytic subunit of a protein kinase, CDK -cyclin-dependent kinase, with a regulatory subunit, cyclin.

     2.Once cyclin-dependent kinases attach to cyclin, the organized compound is in an CDKCs confederation with Cell Cycle stage in Yeast.

B.) 1. It has been revealed that (pRb) Rb protein is answerable for a prime G1.

2.A/B empire of the pocket area and on Rb proteins can be compound on .

3. As a straight reaction, E2F transcription component are extricate by command of Rb.

C) 1. (ALP, ALKP, ALPase, Alk Phos -Alkaline phosphatase is a hydrolysis enzyme organize for detaching phosphate categorys from many variety of molecules, including alkaloids, nucleotides, and proteins.

2.The action of detaching the phosphate categorys is knwn as dephosphorylation.

D. Ans) 1. There are abnormality to the \"two-hit\" regulation for tumor suppressors, such as unquestionable mutations in the p53 gene consequence.

2. p53 modification can purpose as a \"dominant negative,\" explication that a modified p53 protein can intercept the function of ordinary protein from the un-modified allele.