On time crunch fast help appreciated thank you Can anyone

On time crunch! -- fast help appreciated :) thank you!!!

Can anyone help with these two? On the first one I\'m kinda sure that Affinity-redox complex would not be the answer. So to get full credit I feel like i have to remove one of my options i added but idk which. But i could be wrong and maybe all of them are right? For the question after i thought it was only TDI (Two-Dimensional Isoelectrophoresis) but i got it wrong. I tried TDI (Two-Dimensional Isoelectrophoresis) and Using isoelectric focusing, but i still got it wrong. Any help is much appreacited!!!

0.67 1 pts Partial Question 9 Select all correct molecules: In affinity chromatography, what could the beads in the column likely have covalently attached to them? 2+. Na or Ca v Acetylene Ligand Lectins None of the above Affinity-redox complex

Solution

In affinity chromatography the gels are porous supports that are made of sugar- or acrylamide-based polymer resins that are produced in solution (i.e., hydrated) as 50-150µm diameter beads. These beads are of high affinity to the protein. Ligands are covalently attached to the bead polymers.

pI is isoelectric point of the protein at which the protein has no net charge. The charge depends on the pH, At a pH below their pI they carry a positive charge; If pH is above their pI they have a net negative charge. So the proteins are seperated by isoelectric focusing which is 2-D gel polyacrylamide gel electrophoresis or 2D gel electrophoresis