Use of the Compound Light Microscope DATA AND OBSERVATIONS T

Use of the Compound Light Microscope DATA AND OBSERVATIONS The eyepiece contains a glass lens that 10 times (10x) The low-power objective also contains a lens that magnifies 10 Dmes ma total magnification of an obj the wed under low power is 100x. Total magnification is calculated by the cab on of the objective by that of the eyepicce. 1. What is the total magnification of your microscope under low power? (Use the numbers printed on your low-power objective and eyepiece if present.) 2. What is the total magnification of yonr microscope under high power? (Use the numbers printed on your high-power objective and eyepiece.) ANAL WSIS 1. Match the microscope parts with their functions. Write the letter of the function in front of the correct part. a. Allows light to pass through stage diaphragm b. Brings objects into rapid but coarse focus c. Regulates amount of light entering microscope Sage opening d. Is attached to revolving nosepiece and contains a len mirror or lamp capable of 10x magnification eyepiece e. Contains a lens capable of 43x magnification -e low-power objective f.. Supports slide -E high-power objective g. Directs light into microscope revolving nosepiece h. Turns to change the objective positioned over the st coarse-wheel adjustme opening i. Contains a lens capable of 10x magnification fine-wheel adjustment j. Brings objects slowly into fine focus 2. Identify the following statements as true or false. a. Total magnification of a microscope is determined by adding the eyepiece-l magni6ca to the objective-lens magniiluauon. b. The Sne-wheel adjustment must be used to sha focus when using high-power magnific Ten Always look to the side of a light microscope when lowering the objective. d. The eyepiece of a microscope is marked 10x.The high-power objective is marked 50x The boul magnifiq tion is 500x. FURTHER ExpLORATIONS Collect standing water from a variety of such as tee stumps, abandoned automobile tires, o sources tin With microscope, many in the Check rary for books help in the microscopic organisms 2. Research the construction of early microscopes, such as the simple instruments made by van Leeuwenhoek. Determine how a simple microscope is different from a compound light microscope and draw dia showing the optics of each. grams

Solution

1.Total magnification=Power of eyepiece x power of objective

hence magnification under low power is 10x X10x =100x

2.Magnification under high power is 10xX40x=400x.

Analysis;

Microscope parts and function.

C diaphragm

a Stage opening

G mirror or lamp

I eyepiece.

D low power objective.

E high power objective.

H revolving nose piece.

B. coarse wheel adjustment.

J . fine wheel adjustment.

F. stage.

Identify the following statements as true or false.

a. False. Total magnification of a microscope is determined by multiplying the eyepiece -lens magnification to the objective lens magnification.

b. False . The fine wheel adjustment must be used to sharpen focus under low magnification and is used for all focusing under high magnification.

c. True.

d. True.