For toxoplasma gondi you want to understand the pathways tha

For toxoplasma gondi you want to understand the pathways that are involved in the parasite cell cycle regulation. Describe the methodology that will let you identify at the genome-wide level of all possible proteins that may be involved in the cell cycle checkpoints. Describe the experiments that will validate your findings and how the results might provide novel targets for antivirulence based therapies.

Solution

To identify all possible proteins that may be involved in the cell cycle checkpoints at the genome level, the genes encoding these protein are an expression cDNA library will be created. Specific mutations are inserted into the genes by site directed mutagenesis. The mutations should produce a truncated protein which is not functional. These mutated proteins will not carry out cell cycle regulation leading to abnormalities. Hence they can be identified.