Question 6 5 points Save An Your thermocycler is only able t

Question 6 5 points Save An Your thermocycler is only able to reach 70oC, what step of PCR would be affected first? Why would your PCR be unlikely to work? T T T T Paragraph Arial 3 (12pt) E IE T A HTML C55 fr Mashups

Solution

Answer : The step 3 (Primer extension) of the polymerase chain reaction affected the most. This is due to the fact that as all known DNA polymerases (Including the Taq polymerase used in PCR) requires primers (short strand of DNA that serves as a starting point for DNA synthesis) with exposed 3\' OH groups for the purpose of polymerisation/amplificationof DNA.