Sally wants to express her gene of interest in E coli She in

Sally wants to express her gene of interest in E. coli. She inserts her gene of interest into an expression plasmid. Upstream of the gene she inserts the Prinbow box and downstream she inserts a Rho-independent termination sequence. She transforms this plasmid into E. coli, but the E. coli don’t transcribe her gene. What did she do wrong?

Solution

For the transcription of a gene promoter ,coding and terminator are required.RNA polymerase binds on promoter and transcribe coding and terminator.promoter is never transcribe. there is a start site which is a part of coding region and -10 sequence upstream to it is pribnov box at which unwinding takes place first annd -35 sequence upstream is GC box which is also called sigma recognition box at which sigma subunit of polymerase binds.In this case she inserted only the pribnov box but for transcription complete promoter is needed that\'s why her gene might have not transcribe