1 AWhat is the concentration of your DNA solutionugml BWhat

1) A)What is the concentration of your DNA solution(ug/ml)? B)What is the total amount of chromosomal DNA(in ug) from the 0.1ml of cell suspension? C)Assusming you started with 4g liver tissue, what is the yield of DNAzol isolation( ug DNA per g of tissue)? D)Assuming the cow genome is 3 Gb(10^9 base pair) and the average molecular weight of a base pair is 650 Dolton, how many cells did you have in the 0.1 ml cell suspension?

Thermo dsDNA (Factor: 50) #7 A260 (10 mm): 0.601 A260 A280: 2.24 30.0 nglHl 07 Feb 2017 15:04:48 Blank 07 Feb 2017 15:03:49 Measure sample Home Blank Print Measure

Solution

A. concentration of DNA Solution 0.2-0.3ug/ml.

B. total amount of chromosomal DNA 0.03ug/ 0.1 ml

C. 0.03-0.05ug DNA/g

D. 10⁶ cells/ 0.1ml suspension