What is the comparison of annealing temperature vs fracture

Solution

The annealing temperature (Ta) chosen for PCR relies directly on length and composition of the primers. Generally, you should use an annealing temperature about 5°C below the Tm of your primers. The optimal annealing temperature (Ta Opt) for any given primer pair on a particular target can be calculated as follows: Ta Opt = 0.3 x(Tm of primer) + 0.7 x(Tm of product) - 25; where Tm of primer is the melting temperature of the less stable primer-template pair, and Tm of product is the melting temperature of the PCR product.

Fracture temperature is the temperature at which a material changes from one crystal state (allotrope) to another. For example, when rhombic sulfur is heated above 96°C it changes form into monoclinic sulfur. When cooled below 96°C it reverts to rhombic sulfur.

In the case of ferroelectric or ferromagnetic crystals a transition temperature may be known as the Curie temperature.