Assume you are setting up transformations with EColi using s

Assume you are setting up transformations with E.Coli using selective media containing Ampicillin,X-gal, and IPTG.

predict the impact of inserting a very large foreign DNA fragment ( 5+Kb) into pUC18 on the number of plasmids replicated in each cell and the efficiency of transformation as well as the phenotype ( color) of the colonies transformed with the plasmid.

Solution

Copy number refers to the average or expected number of copies per host cell. Plasmids are low, medium or high copy number.

Plasmids vary widely in copy number depending on three main factors: