Homework SourseThe primers we will use for PCR will Acompletely and fully a
The primers we will use for PCR will...
A)completely and fully anneal to a complementary sequence in the yeast genome. If there is no full overlap and annealing, the PCR will not be successful.
B)Will have restriction sites that exist on the vector. Because of this, the primers will not be annealing fully and completely to the genomic DNA
Will both have the same restriction site incorporated. That will ensure proper insertion into the vector, which would be also cut with the same enzyme.
D)Will have some random sequence that does not anneal to anything, right in the middle of the gene of interest. This is to make sure that the restriction enzyme can grab onto something before it can cut the DNA.
E)Incorporate restriction sites that are naturally occuring in the yeast genome
| A)completely and fully anneal to a complementary sequence in the yeast genome. If there is no full overlap and annealing, the PCR will not be successful. | ||
| B)Will have restriction sites that exist on the vector. Because of this, the primers will not be annealing fully and completely to the genomic DNA | ||
| C) | Will both have the same restriction site incorporated. That will ensure proper insertion into the vector, which would be also cut with the same enzyme. | |
| D)Will have some random sequence that does not anneal to anything, right in the middle of the gene of interest. This is to make sure that the restriction enzyme can grab onto something before it can cut the DNA. | ||
| E)Incorporate restriction sites that are naturally occuring in the yeast genome |
Solution
The choice C is correct.
Explanation: For any primer to amplify a target site on a vector, it should be having the same restrictin sites as that of the vector. This is because of two reasons:
Thus, these explanations suggest that choice C is correct.
