For a sequence 5ATCGATCGAT3 what is its complementary strand

For a sequence 5’-ATCGATCGAT-3’, what is its complementary strand? Assuming that each hydrogen bond requires 10 kJ/mol to break, how much total energy is needed to separate these two strands completely?

Solution

~ Remember, A pairs with T and G pairs with C. Go through the original 5 to 3 sequence pairing each A with T and each C with G. Keep in mind that the complementary strand will read from left to right in the 3 to 5 direction. Therefore, the complementary strand starts with 3’ and ends with 5’.

Original strand - 5\'-A-T-C-G-A-T-C-G-A-T-3\'

Complementary strand - 3\'-T-A-G-C-T-A-G-C-T-A-5\'

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