What is the most logical sequence of steps for splicing fore

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium? I. Transform bacteria with a recombinant DNA molecule II. Cut the plasmid DNA using restriction enzymes. III. Extract plasmid DNA from bacterial cells. IV. Hydrogen bond the plasmid DNA to nonplasmid DNA fragments. V. Use ligase to seal plasmid DNA to nonplasmid DNA I, II, IV, III, V II, III, V, IV, I III, II, IV, V, I III, IV, V, I, II IV, V, I, II, III Assume that you are trying to insert a gene into a plasmid. Someone gives you a preparation of genomic DNA that has been cut with restriction enzymes X. The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, . but not for X. Your strategy should be to insert the fragments cut with restriction enzyme X directly into the plasmid without cutting the plasmid. cut the plasmid with restriction enzyme X and insert the fragments cut with restriction enzyme Y into the plasmid. cut the DNA again with restriction enzyme Y and insert these fragments into the plasmid cut with the same enzyme. cut the plasmid twice with restriction enzyme Y and ligate the two fragments onto the ends of the DNA fragments cut with restriction enzyme X. cut the plasmid with restriction enzyme X and then insert the gene into the plasmid.

Solution

11. Answer is C: Extract plasmid DNA, Cut the plasmid DNa, Hydrogen bond between plasmid and DNA, SEAL DNA USING LIGASE, Transformation into bacteria

12. Answer is C: Cut DNA with Y and insert into plasmid that is already digested with the Y