Class Dala YEPD plati Strains of S ra in r op muti muting ty

Class Dala YEPD plati Strains of S ra in r op muti, muting type type pln) Plate Strui, r o mutiny Struts of muting t pe ple al a 2 a a a a

Solution

a). If the genotype of strain 4 is HIS1; HIS6; his5; HIS2

The genotype of strain 2 can not be estimated based upon this data. Based upon the given information only the genotype at locus HIS5 can be determined, which is HIS5.

b). The colony color of strain no. 6 is pink, which might be indicative of the mutation at the locus ade5. Due to the mutation in ade5, its protein is not formed resulting in the accumulation of the pink colored intermediate.

c). Following complementation, the wild allele, ADE5 was complemented by the mating pair. Therefore, the protein is formed and the adenine is synthesized. When there was no complementation, the adenine biosynthetic pathway is not initiated, thus, no pink intermediate.