Which of the following gives you a threedimensional image a

Which of the following gives you a three-dimensional image?. a. Differential interference microscopy b. Scanning electron microscopy c. Confocal microscopy d. Phase contrast

Solution

SEM- SEM gives detailed three-dimensional and topographical imaging and the versatile information collected from different detectors. SEMs are also easy to operate with the proper training and advances in computer technology.

Confocal MIcroscopy give better resolution and contrast by adding a spatial pinhole placed at a confocal plane to remove the light which is out of focus which enables the detection of three-dimensional structures from sets of images obtained at different depths within a thick object.

Differential Interference Contrast can detect the 3D structure but it is not the recommended teqnique to detect 3D structure beacause it is not accurate for 3D analysis.The enhanced areas of light and shadow might add distortion to the appearance of the image which make image unclear.