You are working in a lab and studying the characteristics of

You are working in a lab and studying the characteristics of a particular protein. You have paid for a commercial company to synthesize the protein for you. Somehow, they have mistaken your instructions and synthesized it with the amino acids in the reverse order. A friend of yours says that since the same amino acids were used, it should be fine to characterize this protein. What do you think?

Solution

Answer:

The protein synthesized in reverse order will have different configurations and properties compared to the right one.

Proteins in reverse order will have different amino acids at N terminal and C terminal which affects the biological function of the protein.

Under such situations we should resynthesize the right protein for characterization.