Part A Selecting the Most Appropriate Method of Viewing Thi
Solution
Transmission Electron Microscope (TEM)
answer :
Viewing ribosomes (20nm) within a bacterial cells;
confirming the 9+2 microtuble arrangement in a eukaryotic flagellum;
viewing a cross section of poliovirus (30nm);
view the layers of the gram-negative cell wall in cross section
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light microscope
answer :
Examining a clinical specimen, such as a sputum smear for the presence of bacteria; examining a blood smear for the presence of malarial parasites
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Scanning Electron Microscope (SEM)
answer
Viewing the 3-D shape of a protozoan and the arrangement and distribution of cilia on its surface; determining the 3-D shape and arrangement of bacterial cells
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answer
Bacterial Plasmids (20nm)<Mastadenovirus (30nm) < Gram-pos bacteria (6µm) < Giardia lamblia protozoa (7µm) < Cionorchis sinensis trematode (5mm)
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lens that is closest to the slide and provides initial magnification of a specimen
objective lens
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used after initial focusing to sharpen the image------------fine focus knob
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platform on which side is placed for viewing-----------stage
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used to focus the light from the illuminator onto the slide-------condenser lens
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used for initial focusing should never be used when the high power objective
coarse adjustment knob
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controls how much light from the illuminator reaches the specimen-----------
diaphragm
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