1aSuccessful bacterial DNA replication does NOT use ligation

1aSuccessful bacterial DNA replication does NOT use…
   ligation of DNA nicks
   priming new synthesis using RNA polymerase
   a bidirectional fork
   chaperones to stabilize single strand DNA

1bWhich process does NOT use a specific nucleotide sequence for activity?

   transcriptional termination
   replication initiation
   translational termination
   helicase unwinding

1c Place the following steps of DNA replication in order:

    1RNA priming
    2Elongation
    3Helicase loading
    4Origin melting

   1, 2, 3, 4
   1, 2, 4, 3
   4, 3, 1, 2
   3, 4, 1, 2

Solution

A successful bacterial replication comprises of a bidirectional fork, DNA ligase to cause ligation of nicks, and also SSB proteins, which are chaperones to stabilize single-stranded DNA.

Transcription termination uses a sequence-specific signal to terminate the process and dissociate RNA polymerase from the template.

Replication initiation occurs at the origin of replication site, also known as ori site. This ori site has a specific conserved sequence of nucleic acids.

Translational termination occurs at a site corresponding to the stop codon in the mRNA. The stop codon is a 3-letter nucleic acid specific site.

In DNA replication, the following steps occur in order:

Origin melting, Helicase loading, RNA priming, Elongation