The following electrophoresis gel resulted from a study of t

The following electrophoresis gel resulted from a study of the effects of three restriction endonucleases (Pstl, EcoRI, and Sall) both alone and in combination on the plasmid pNEW. Labels above the gel indicate what went into each well; numbers to the right are the estimated sizes, in kilobases (kb), of the fragments comprising each of the bands. Your objective is to use these data to construct a restriction map for pNEW by following the steps presented Examine the gel and estimate the total size of the plasmid in kilobases (kb). 3.2b 3.6b 4.0b 4.4b

Solution

14. D) 4.4 Kb

Single cut produce 4.4 kb length plasmid in lane 1, 2 and 4. When we sum up the individual weights of fragments it equals to 4.4 kb in each lane.