help with molecular biology short answers 8 In Travers and B

help with molecular biology short answers

8. In Travers and Burgess\' experiment looking at the function of the sigma subunit with nucleotides labeled with either C-14 on P-32, what was their independent variable variable on the X-axis of their dose response curve 9, when Travers and Burgess used c-14 labeled nucleotides, which process(es) of transcription did they measure? 10. If one is using radioactive es (e.g., ATP, UTP, GTP or CTP) to label RNA, which nucleotid atom in the nucleotide is radioactive in order to measure transcription initiation only Be specific. 11 Which antibiotic binds RNA polymerase to block transcription initiation? Spelling counts. 12. What does FRET measure? 13. In order for FRET to work, the two fluorescent molecules must have something in common. What of one molecule must equal what of the second molecule? 14. Fill-in-the-blank. The presence of ndicates that not all transcription initiation events end up finishing transcription.

Solution

If one using, radioactive nucleotides (e.g. ATP, UTP, GTP or CTP) to label RNA, which atom in the nucleotide is radioactive in order to measure transcription initiation only? Be specific

Answer: UTP

Transcription is the process of making RNA from DNA. DNA and RNA both are having nitrogenous bases adenine (A), guanine (G) and cytosine (C); but thymine (T) is specific for DNA whereas, uracil (U) is specific for RNA. Hence, UTP in the nucleotide is radioactive in order to measure transcription initiation only.

Which antibiotic binds RNA polymerase to block transcription initiation? Spelling counts

Answer: Rifampicin

Rifampicin inhibits DNA-dependent RNA synthesis by inhibiting DNA-dependent RNA polymerase.

Fill in the blank. The presence of ……………… indicates that not all transcription initiation events end up finishing transcription.

Answer: Introns

An intron is a nucleotide sequence, which is removed by RNA splicing during maturation of the final RNA.