For a different experiment you are growing up Saccharomyces

For a different experiment you are growing up Saccharomyces cerivisiae instead of E.coli. The measured OD is 0.89. A) In order to calculate the concentration of the Saccharomyces cerivisiae culture, can you use the equation that you use to calculate the concentration of an E.coli culture? If yes or no, give a detailed explanation. B) Does E.coli or Saccharomyces cerivisiae have a shorter generation time? Give a detailed explanation!

Solution

A. The equation used to calculate the concentration of saccharomyces cervisea culture is same as bacteria.Normally Beers law is used to calculate concentration from absorbance.Beers law states that concentartion is directly prpotional to absorbance.

B. E.coli has a shorter generation time than yeast. E.coli generation time is 20 miutes and yeast generation time is 80 minutes. Time required for the organism to doubling in a cetain time period is called generation time.