List at least three differences between a cDNA library and a

List at least three differences between a cDNA library and a genomic library. You have an oligonucleotide probe that hybridizes to part of gene A from a eukaryotic cell. Indicate whether a cDNA library or a genomic DNA library will be more appropriate for use in the following applications. A) You want to study the promoter of gene A. B) Gene A encodes a tRNA and you wish to isolate a piece of DNA containing the full-length sequence of the tRNA. C) You want to find both gene A and the genes located near gene A on the chromosome. D) You want to express gene A in bacteria to produce lots of protein A.

Solution

A collection of clones containing all the DNA sequences carried by an individual is called a genomic library. For example, identifying a mutant bacterial gene by plasmid library transformation requires a genome library of the wild type organism, a culture of the mutant, transformation of the mutant with the plasmids carrying the genomic library and minimal media with the appropriate antibiotic for selection.

A cDNA library is defined as the \"mRNA is going to form DNA\" from a species cell in complimentary to the mRNA in the presence of \"reverse transcriptase\" so that this mRNA sequences are kept under a sequencing library to promote coding for cDNA