The first proteins to elute on a gel filtration column are t

The first proteins to elute on a gel filtration column are the______ proteins in the sample.

a.) smaller

b.) larger

c.) negative

d.) positive

The explanation from the question above is:

a.) larger proteins become trapped in the gel beads, allowing smaller proteins to elute first

b.) larger proteins have shorter path length through the column compared to the smaller proteins

c.) smaller proteins travel a shorter distance through the column as compared to larger proteins

d.) smaller proteins are excluded from the interior of the gel beads so they have less available column space to travel compared to the larger proteins

Solution

answer is B larger

b.) larger proteins have shorter path length through the column compared to the smaller proteins