Can someone help me out with this homework It has four parts
Can someone help me out with this homework. It has four parts and I am unsure of the answers. Thanks.
Select the correct 18mer (18 bases long) forward primer to amplify the DNA sequence comprised between the two bases indicated in red color and underlined.
Select the correct 18mer (18 bases long forward primer to amplify the DNA sequence comprised between the two bases indicated in red color and underlined 5\'GAATTCATGCGTAGATCGATGATCATGATGCGATGATGATGATGATGCCGTATAGATGTATAAATGATA GATT TTGACACGCATATAGACATAGTGGATTGGGATAGATCGTGATGATGGT TCTCTCGATTTAGGTAT TAGATGTAGGATAG ATTCCCGGG 3\' FORWARD PRIMER a GAATTCATGCGTAGATCG o ATGCGTAGATCGATGATC CGTAGATCGATGATC o ATCCGTAGATCCATGATC O TTT CGTAGATCGATGATC o ATGCGTAGATCGGCGCGCG ATGCG O ATG CGT o ATGCGTAGATCSolution
Answer A. Forward Primer GAA TTC ATG CGT AGA TCG
Answer B reverse primer GAA TCT ATC CTA CAT CTA
Answer C. Given sequence is the sequence of the mRNA because it has Uracil instead of thymine so when we design the primer we have to replace U to T.
Forward primer- ATC GAT GAC GAT GGA CGA
Answer D. Given sequence is the sequence of the mRNA because it has Uracil instead of thymine so when we design the primer we have to replace U to T.
None of the options are right
Answer will be - AT CGC TCT ATC TCG TCT TTT TCG
