The graduate student uses the genetically manipulated mice a

The graduate student uses the genetically manipulated mice and performs the following experiment. One genetically manipulated mouse receives soybean extract in their food for 3 weeks while another receives normal food. Samples of liver tissue from both mice are taken and analyzed as follows: The graduate student proposes that their experiments support the idea that soybean extract alleviates the diabetes condition by establishing normal levels of active GSK3. Thus leading to normal levels of glycogen storage. You on the other hand have difficulties with their conclusions. Explain why. (Explain why their conclusions are incorrect and also write the correct conclusions based on the current data).

Solution

Role of gsk 3 in insulin stimulated glucose transport and glycogen synthase activation. Here , in this data mice with soyabean diet show thick band than normal mice. In diabetic patient dysregulation of gsk3 occur. This enzyme inactivated by phosphorylation . Here, the band show higher amount of phospho gsk 3 means inactive form of this enzyme. Gsk3 inhibit glycogen synthase via its phosphorylation. Here, inactivation of gsk3 leads to the inactivation of phosphorylated glycogen synthase in soyabean extract food sample. So, conclusion of this experiment is soyabean extract doesnt produce diabetic condition because diabetic patient show increase in gsk3 activity not phospho gsk3 activity. Here , glycogen deposition will increase due to active form of glycogen synthase.