NEED HELP IN PUTTING THIS AMINO ACID SEQUENCE INORDER An 8me

NEED HELP IN PUTTING THIS AMINO ACID SEQUENCE INORDER

An 8-mer oligopeptide has the following amino acids:

A D E E F M W Y (two Es are present)

Here is your evidence:

1. Edman degradation produces A

2. CNBr produces a 5-mer and a 3-mer that contains W

3. Chymotrypsin produces two dipeptides and a tetrapeptide. The tetrapeptide has a total net charge of -4 at pH 12.5.

4. Staphylococcal protease in phosphate buffer produces a tetrapeptide (4-mer), dipeptide, free D, and free W.

5. Staphylococcal protease in acetate buffer produces a pentapeptide (5-mer), dipeptide, and free W.

Put in the sequence of the 8-mer (use caps here) i.e. ABCDEFGH

Solution

The sequence should be ADEFYMEW. CNBr hydrolysis peptide bonds at C terminus of the methionine residues . So it must be A _ _ _ _ M _ W or  A _ _ _ _ M W _ A is in 1st position because 1st group is degradaded in edman degradation - Nterminal group is degraded. 3 aminoacids are acted by chymotrypsin . They are F, W , Y. It produces 2 dipeptides and one tetra peptide . So must be in the form A_ _ F Y M _ W. Protease cleaves specifically on Asp or glutamate residues . So it must be ADEFYMEW. Protease in acetate buffer cleaves glutamate residues. Hence the order should be  ADEFYMEW.