Show all work clearly Please Protein z can bind to two ligan

Protein z can bind to two ligand named v and w. The two complexes have the following Kd values: Kd(zv) = 300 nM, and Kd(zw) = 0.3 mu M. Which ligand has the highest affinity for protein Z? Explain.

Solution

The smaller the K_D value the greater the affinity of the antibody for its target.At equilibrium, the rate of [antibody] [antigen] complex formation is equal to the rate of dissociation into its components [antibody] + [antigen]. The measurement of the reaction rate constants can be used to define an equilibrium or affinity constant (1/K_D).

So, reaction rate of V=1/Kd=1/300(300 nm=0.3 micrometer.)

reaction rate of W=1/Kd=1/0.3

so, both ligand have same affinity towards protein Z.