Q Assume that conversion of one mole of glycine to one mole

Q) Assume that conversion of one mole of glycine to one mole of acetyl-CoA consumes one mole of ATP. How much ATP (net) can the organism produce from a mole of glycine?

A) The strain is a mutant that cannot couple electron or proton transfer from FADH2. How many ATP are produced?

B) The mutain strain from A is grown under anaerobic conditions with nitrate as its terminal electron acceptor. Assume 2 moles of ATP per mole of NADH2. How many ATP are produced?

*it is a 3 part question...Thank you so much for the help!

Solution

We know that every acetyl-CoA yields 3 NADH + 1 FADH₂ + 1 GTP (=ATP) during Krebs cycle. Also, we know that 1 NADH yields 3 ATP and 1 FADH₂ yields 2 ATP.

Therefore, ATP produced from one mole of acetyl CoA = 3×3 + 1×2 + 1 = 12 moles.

Given that conversion of one mole of glycine to one mole of acetyl-CoA consumes one mole of ATP. Therefore, net ATP produced from one mole of glycine = 12-1 = 11 moles

A) If the strain is a mutant that cannot couple electron or proton transfer from FADH₂:

Total ATP produced = 3×3 + 1×0 + 1 = 10 moles, and net ATP produced will be 10-1 = 9 moles

B) If the mutain strain from A is grown under anaerobic conditions with nitrate as its terminal electron acceptor, such that there are only 2 moles of ATP per mole of NADH:

Total ATP produced = 3×2 + 1×0 + 1 = 7 moles, and net ATP produced will be 7-1 = 6 moles