Give two possible strategies that can be used to design a va

Give two possible strategies that can be used to design a vaccine against an infectious agent?

Solution

Generation of antibodies against an antigen can be done in following mentioned methodologies:

1.Natural immunization: This represents the basic immunological method of antibody generation against an antigen. In this method, the antigen is applied to a host and allowed to react in the body. By the virtue of humoral immune response, the B-cells of the host slowly produces antibodies against the antigen. These antibodies can be extracted from the blood by centrifugation to separate serum and purified by affinity chromatography. This method is generally utilized to generation antibodies against multiple antigens.

2.In silico designing and peptide generation: When a particular type of antigen is considered lethal for an organism, it is not directly made to immunize the host but a safer bioinformatics approach is used. In this approach, the structure identification is performed and online databases are searched for antibody structures compatible to them. The best matches are screened and synthesized in vitro for analysis of immune response in the body against the antigen.

Thus, these two common methods are utilized for antibody generation against an immunogen/antigen or infectious agent.