1 You need 15mM Tris in a volume of 14mL You have 200mM Tris

1. You need 15mM Tris in a volume of 14mL. You have 200mM Tris in a stock bottle on your bench. ¥ What volume of 200mM Tris do you need to make the 14mL of 15mM Tris? ¥ What volume of water do you need to make the 14mL of 15mM Tris?

Solution

we know that S1V1=S2V2

S=Strength, V=Volume

15 x 14 = 200 x volume required

volume required= 15 x 14/ 200=1.05 ml

so to make 14 ml of 15mM Tris we need 1.05 ml of 200mM Tris and 12.95ml water.