Using the numbers on your data sheet calculate the Rf for ea

Using the numbers on your data sheet, calculate the R_f for each identifiable band. Which bands, if any, appeared/disappeared/changed appearance between the two plates? Use their color and R_f when referring to them. The expectation is that the chlorophylls in your sample arc converted to pheophytins when treated by acid. Your results may, or may not, agree with this expectation. Analyze your results objectively, and state whether your results are in line with this expectation, or not. If there are unexpected similarities or differences between the two plates, suggest what may be causing them.

Solution

Answer:

\"Pheo\" means \"dusky\"

\"Phyte\" means \"vegitable\"

Biochemically, pheophytin is defined as a chorophyll molecule that lacks a central Mg2+ ion.

Pheophytin can be produced from chlorophyll by treatment with a weak acid. by giving a dark bluish waxy pigment.

If you have two plates, where chlorophylls converted into pheophytin, it could be

i) Due to presence of purple bacteria, as pheophytin is the first electron carrier intermediate in the photoreaction center (RC P870) of purple bacteria. where excitation of the bacteriochlorophylls (Chl)₂ occurs

ii) due to electron transfer pathway of photosystem II , as pheophytin is the first electron carrier in plants.

It can be proved by using electron paramagnetic resonance (EPR), where you can show that pheophytin is reducable and they act as primary electron acceptor.