2 Using standard molecular techniques you isolate the gene t

2. Using standard molecular techniques, you isolate the gene that seems to be responsible for susceptibility to the drug. The sequence of the dominant and recessive alleles is shown below. The sequence of the propositus is also shown (two alleles, as the individual is human and thus diploid). Based on this data, will the propositus be able to be cured with the drug? Why or why not? (1.5 points) Dominant allele 5\'-AATAGACAGTACAGTAGGGA AGAGTT TAG TAG-3 Recessive allele 5.AATAGACAGTACAGTAGGGA AGA GTTTAGTAG-3 AGAGTTTAGTAG-3\' Propositus allele 1: 5\'-AATAGACAGTACAGTAGGGA Propositus allele 2: 5\'-AATAGACAGTACAGTAGGGA AGAGTTTAGTAG-3

Solution

The given nucleotide sequence is the coding strand so when the protein is translated from the sequence it will as

AAT AGA CAG TAC AGT AGG GAC CGA GAG TTT AGT AG

N      R       Q      Y       S      R       D     R        E       F      S   

AAT AGA CAG TAC AGT AGG GAC TGA GAG TTT AGT AG

N       R      Q       Y      S      R        D     Stop     

Each drug has different property when it comes to binding to the protein which we can infer from the structure of the drug. Some drugs have made up of only hydrophobic residue which gives them property to bind hydrophobic domain of the protein. Some drugs have polar residue such as Oxygen or some positive charges metals which give them property to bind the hydrophilic domain of the protein

When the gene got mutated, codon CGA was replaced with TGA.

Dominant gene got translated into full-length protein. Due to mutation in recessive gene stop codon (Non-sense mutation) generated in place of arginine amino acid codon, lead to the translation of gene into truncated protein.

Drugs have the specific binding site in each protein and it varies from drugs to drugs. If anyhow those sites are mutated, then the drug is not able to bind to its specific bind site.

When the given gene is mutated into recessive gene, drugs binding site lost due to the generation of truncated protein. But in case of dominant gene, it has intact binding site for the drug, which makes the drug to bind to the product of the dominant gene