Assume that you have a 20 M glycerol stick solution You need

pirections: Assume that you have a 20 M glycerol stock solution. You need to make 5 different concentrations of solutions for your experiment. For the experiment you will need to make 50 ml of each concentration of glycerol. Use the formula: C,V C,V, Final Concentration of Amount (MI) of 20 Amount of Water Final Volume of the Solution M Glycerol Stock (V2 Solution Solution (C) Needed 0 M glycerol 500 ml 2.5 M glycerol 50 ml 30 ml 5 M glycerol 7.5 M glycerol 50 ml 10 M glycerol 50 ml Show your calculations for each solution. Remember: As long as the units are the same on both sides of this formula it doesn\'t matter which units you use, they will cancel out. 0 M glycerol 7.5 M glycerol C, nal concentration souri 2.s Volume of 20 M glycerol needed? Volume of H20 needed? 2.5 M glycerol 10 M glycerol 5 M glycerol

Solution

The formula is C₁V₁ =C₂V₂

The concentration of the stock solution of glycerol, C₁ is always the same.

Question 1: Preparation of 7.5 M glycerol solution

C₁ = 20 M

V₁ = ?

C₂ = 7.5 M

V₂ = 50 ml

Substituting the equation,

20 X V₁ = 7.5 X 50

V₁    = (7.5 X 50)/20

      = 18.75 ml

Quantity of water needed to make up the solution = Total volume of the solution - Volume of stock solution

      = 50 - 18.75

= 31.25 ml

Question 2: preparation of 2.5 M glycerol solution

C₁ = 20 M

V₁ = ?

C₂ = 2.5 M

V₂ = 50 ml

Substituting the equation,

20 X V₁ = 2.5 X 50

V₁    = (2.5 X 50)/20

      = 6.25 ml

Quantity of water needed to make up the solution = Total volume of the solution - Volume of stock solution

      = 50 - 6.25

= 43.75 ml

Similarly, other values can be worked out

C ₂

V_{1 = (}C₂*V₂)/ C₁

V₂ - V₁ = 50 - V ₁

0

0

0

2.5

6.25

43.75

5

12.5

37.5

7.5

18.75

31.25

10

25.0

25.0

C 2	V1 = (C2*V2)/ C1	V2 - V1 = 50 - V 1
0	0	0
2.5	6.25	43.75
5	12.5	37.5
7.5	18.75	31.25
10	25.0	25.0