Which one?
You use the streak-plate method to obtain a pure culture and observe individual colonies following incubation. Consider a single bacterial colony. All of the following statements about the colony are true EXCEPT: Nutrients slowly diffuse through the agar and feed the bacteria in the colony, and similarly, wastes from the colony slowly diffuse away. All cells in the colony are genetically identical, except for any mutations that might have arisen during binary fission. Cells at the edges of the colony are likely experiencing a higher oxygen concentration than those in the middle of the colony. All cells in the colony are expressing the same set of genes and are performing the same metabolic functions. Cells at the middle of the colony are probably growing less actively than cells at the edges of the colony.
Ans.) In the given options, only the 4th option do not seems appropriate.
(In a bacterial culture, all cells have same set of genes but it is not necessary that they do not perform the same metabolic activity.)