In biology studies if we have any colored solutions that mea

In biology studies, if we have any colored solutions, that means we can measure the INTENSITY of the COLOR by spectrophotometry and relate it to the concentration of that component (solute) at any given time. TRUE OR FALSE? Explain.

What is the range of numbers for absorbance in spectrophotometry? __ to ____ and What number is beyond limit?

Explain the relationship between optical density and absorbance value.

Solution

True. Spectrophotometry uses photometers that can measure a light beam\'s intensity as a function of its color (wavelength) known as spectrophotometers.It is used to measure the transmttance of solutions. When a solution concentration is high, less light passes through it and vice versa.

range of numbers for absorbance in spectrophotometry is 0 to 1.

Any value above 1 is beyond limit.

Optical density is directly proportional to absorbance value. The optical density of a substance is the logarithmic ratio of the intensity of transmitted light to the intensity of the incident light passing through the substance.