List the order of elution of these amino acids 1 Mixtures of

List the order of elution of these amino acids:

1. Mixtures of amino acids, which are composed of Tyr, Cys, and Arg, are run on a DEAE column at a pH of 10.

2. Mixtures of amino acids, which are composed of Lys, Glu, and Ile are run on a carboxymethyl column at a pH of 7.0.

1. Elution order of amino acids on a DEAE column (positive charge binds to negative charge of amino acids)

2. Elution order of amino acids on a Carboxymethyl column (negative charge binds to positive charge of amino acids)

Order of amino acids at pH 10 on DEAE column	overall of charge of amino acids pH 10
1.Tyrosine	-1
2. Cysteine	-1
3. Arginine	+1