You have successfully expanded the DNA code by adding the un

You have successfully expanded the DNA code by adding the unnatural bases dNaM and d5SiCS which can base fair to form a Watson-Crick like structure. a) What interactions are responsible for stabilizing the dNaM-d5SICS base pair? b) Will this be more or less stable than an A-T base pair? c) Will the helicase in question 2 above be able to unwind the following double stranded DNA sequence: GGT(NaM)ATT ?

Solution

Answer:

(a) Hydrophobic forces stabilize the unnatural base pairs and hence the hydrophobic interactions are responsible for stabilizing dNam-dSICS base pair.