PURPOSE This assignment is designed to provide you a first

PURPOSE – This assignment is designed to provide you a first hand experience how scientists use bacterial genetics to “discover” how genes participate in interesting microbial phenotypes. To allow you a sense of discovery of the unknown, you will read reviews and primary literature to identify phenotypes that currently are not completely understood. You will utilize the principles of bacterial genetics covered in class to design experiments to connect genes to phenotypes.

1. Describe your phenotype of interest

2. Justify the utility of understanding the phenotype of interest

3. Describe the genes already known to participate in this phenotype (if any)

4. Identify potential missing portions of regulation for the phenotype

5. Describe the genetic screen – include mutagenesis strategy, the rationale for the mutagenesis strategy, how to isolate the appropriate mutant, and indicate the feasibility of your approach (how likely it can be done by one person in one yea

6. Define the controls

7. Indicate a strategy to identify the mutation that alters the phenotype

8. Suggest additional experiments that would allow you to understand how the mutation you have identified regulates the phenotype of interest

What other information you need?

Solution

1.The phenotype will be mRNA level of a gene which is present in bacteria where mRNA is defined as the large family of RNA molecules which help to convey genetic information from DNA to the ribosome,.