You will be preparing 300 ml of media You need to dilute thi

You will be preparing 300 ml of media. You need to dilute this 10% stock of NaCl, the 20% stock of K_2HPO_4 the 1.5M MgSO_4 and the 10 mg/ml vitamin B1 to prepare your media. You will need 1.5% NaCl and 2% K_2HPO_4 Calculate how much stocks you need to add. Fill in the table below.

Solution

Ans:

45 milliliters of 10 percent NaCl

30 milliliters of 20 percent K2HPo4

100 milliliters of 1.5 molar MgSo4

3 milliliters of 10 mg/ml Vitamin B

122 milliters of water