It would be much appreciated if you can help me with the rec

It would be much appreciated if you can help me with the recipe of the buffer above. Thank you.

You need to make the following cell medium: DMEM, 10% fetal bovine serum, 1x penicillin/streptomycin, 1x glutamine. How much of each would you add to make 500 total mL of media? __mL DMEM ___ mL fetal bovine serum __mL 100x penicillin/streptomycin __ mL 100x glutamine

Solution

The folowing concentrations are required:

1. we need 430 mL of DMEM

2.Moreover we need 50 mL of heat inactivated FBS

3.5 mL of Penicillin/Strep

4.5 mL of gluatamine