why do chloroplasts isolated from land plants become unstabl

why do chloroplasts isolated from land plants become unstable and lose activity?

Solution

Chloroplasts were stored for 1 year with about insignificant losses of photochemical activity. Chloroplasts and newly isolated chloroplasts manifest homogeneous rates of deterioration at 10 °C. Unstable chloroplasts did not convey as excessive retention of activity throughout low temperature storage as unpurified chloroplast adjournment. Excessive retentions of activity were noticed with chloroplasts from separate plant species, as well as with separate oxidants. The activities of sub-trial that were thawed and frozen in a standard way consent within 5%. Decline during lyophilization of chloroplasts transpired mainly, during the early period of drying, and was reduced by sucrose. The unstable of chloroplasts lyophilized in storage assorted straightly with the degree of desiccation and retroverted with the storage temperature; small-scale deterioration transpire in thoroughly dried chloroplasts throughout lengthy storage at or below 5 °C.