What would be the purpose of adding trypan blue to a live mo

What would be the purpose of adding trypan blue to a live mount of brine shrimp? What about adding trypan blue and acetic acid?

Solution

Healthy cell of brine shrimps exclude tryptan blue finally eliminate tryptan blue without being stained so that health replicated brine shrimps are \"colorless\" but unhealthy or damaged cells may take “tryptan blue” and attain color. Therefore, tryptan blue is going to differentiate different kind of healthy and unhealthy brine shrimps where as acetic acid is a powerful cytotoxic agent, when poured onto the cells they are going to acts as cytotoxic on artemia amoebocytes