I would really appreciate some help with these Microbioloy l

I would really appreciate some help with these Microbioloy lab questions. Help will be rated. Thanks

1.)Why is it recomended that you use the broth (regardless of medium) with the most growth to serve as the \"standard\" for scoring a \"3(for abundant growth)\"?

2.)Why is it important to use the uninoculated controls for each medium to serve as the \"standard\" for scoring a \"0(no growth)\" in their respective tubes?

3.)What does comparing growth of a given organism in the three media tell you? (That is, are you gaining information primarily about the organism or about the medium?)

4.)What does comparing growth of the four organisms in a given medium tell you? (Again, are you gaining information primarily about the organism or about the medium?)

5.)Suppose after performing this experiment, you examine th growth of two different organisnms in Medium X. Both show growth and Organism 1 has produced less turbidity than Organism 2. Explani how it could be possible that Organism 1\'s needs are actually better supplied by the medium than Organism 2\'s, yet there is less turbidiy.

Solution

1.growth in broth is usrf as a standard for inoculating. Broth helps in maintaining optimal concentration of bacteria and thus helps us to have umiform growth on solid media.

. 2. Uninoculted tubes serve as negative control. They help us to know that media is sterile.

3.growth on 3 different media tells us about the characteristics of the organism. Each media has different composition and help us to visualize characters like colony morphology, fermentation, hemolysis etc.

4.we gain information about the organisms. Not the media.

5. various factors are responsible for this scenario. The optimum temperature might not be maintained or the growth rate of organism 1 is slower tgan organism 2. Organism 2 may be producing inhibitory substances which inhibit the growth of organism 1