Homework Sourse2 is Pour 15 mL of glycerol top up to 30 mL with water The q
#2 is Pour 15 mL of glycerol, top up to 30 mL with water
The question is... You will be asked to make 20 ml of 10X loading dye. Using the information above,provide a recipe for 20 ml of 10X loading dye. How much of each of the two dyes willyou weigh out? How much of the glycerol solution that you made in question #2 will you be using?
Solution
Answer:
The composition of 10X (20ml) Loading dyes:
As per the given condition in question #2, 15 mL of glycerol (assumed as 100%) is diluted with water to make the final volume of total 30 mL glycerol. It means in 15 mL of glycerol, 15 mL of water is added.
Thus, the concentration of this glycerol is calculated using the formula: N1V1=N2V2
where as N1 = Initial concentration of glycerol (100%); N2 = Final concentration of glycerol (unknown); V1 = Initial volume of glycerol (15 mL); V2 = Final volume of glycerol (30 mL)
Thus, 100 x 15 = N2 x 30
N2 = 50%
And if we have 50% of glycerol as stock, we can make 30% of glycerol by adding the desired volume of water OR we can the calculate the amount of 50% glycerol need to be added in 20 ml (10X) loading dye by using the folmula: N1V1= N2V2
where as N1 = Concentration of glycerol in stock (50 %); N2 = Desired concentration of glycerol (30 %); V1 = Required volume of glycerol (unknown); V2 = Final volume of glycerol in solution (12.5 mL)
50 x V1 = 30 x 12.5
V1 = 7.5 mL
Thus, when we are using 7.5 mL of 50 % glycerol in the above mentioned composition of 10X loading dye, the amount of water will be calculated as: [20ml-(7.5 mL + 1.25 mL)] = 11.25 mL.
Thus now the composition of 10X (20ml) Loading dyes:
