Why does the cytochrome c oxidase experiment include 2 sets

Solution

Answer:The cytochrome c oxidase experiment include 2 sets of blanks are Assay Buffer and Enzyme dilution Buffer solutions.

Cytochrome c oxidase is located on the inner mitochondrial membrane that divides the mitochondrial matrix and it has been used as a marker for this membrane.cytochrome c oxidase assay used for determination of cytochrome c oxidase activity in soluble and membrane bound mitochondrial samples.

The colorimetric assay in this kit is based on observation of the decrease in absorbance at 550 nm of ferrocytochrome c caused by its oxidation to ferricytochrome c by cytochrome c oxidase.

Assay Buffer is used to keep the pH constant.

Enzyme Dilution Buffer is used for making dilutions for restriction enzymes.