You are provided with a chinese hamster ovary cell line CHO2

You are provided with a chinese hamster ovary cell line CHO25 which expresses cell surface receptors for hormone X.

Briefly describe two experimental techniques which would allow you to detect the presence of hormone X-receptor-specific mRNA in the CHO25 cells described in the previous question.

Solution

2. In-situ PCR amplification

You are provided with a chinese hamster ovary cell line CHO25 which expresses cell surface receptors for hormone X. Briefly describe two experimental techniques

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