How would you calculate the volume of buffer to add to a tab
How would you calculate the volume of buffer to add to a tablet that is 9000 FCC units, to make a solution at 1000 units/mL concentration?
Solution
strength of the given tablet - 9000 FCC units
To make it in a solution form at 1000 units/mL concentration, 9000 unit tablet suspended in 90 mL of the buffer
because 9000 units/90 ml will give 1000 units/ml conc.
Grind the tablet to a finely ground powder in a mortar and pestle
Suspend or dissolve this powder of 9,000 units to 90 mL with buffer, including mortar and pestle rinses.
Solution may be a little cloudy because of the undissolved binder but now has the required concentration of
1000 units/ml
